|
|
| Line 1: |
Line 1: |
| [[Image:1jwf.gif|left|200px]] | | {{Seed}} |
| | [[Image:1jwf.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1jwf| PDB=1jwf | SCENE= }} | | {{STRUCTURE_1jwf| PDB=1jwf | SCENE= }} |
|
| |
|
| '''Crystal Structure of human GGA1 VHS domain.'''
| | ===Crystal Structure of human GGA1 VHS domain.=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-interacting proteins) are critical for the transport of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ADP-ribosylation factor (ARF), and clathrin. The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster dileucine (ACLL) sequences. Here we report the X-ray structure of the GGA1 VHS domain alone, and in complex with the carboxy-terminal peptide of cation-independent mannose 6-phosphate receptor containing an ACLL sequence. The VHS domain forms a super helix with eight alpha-helices, similar to the VHS domains of TOM1 and Hrs. Unidirectional movements of helices alpha6 and alpha8, and some of their side chains, create a set of electrostatic and hydrophobic interactions for correct recognition of the ACLL peptide. This recognition mechanism provides the basis for regulation of protein transport from the TGN to endosomes/lysosomes, which is shared by sortilin and low-density lipoprotein receptor-related protein.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11859376}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11859376 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_11859376}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 34: |
Line 38: |
| [[Category: Wakatsuki, S.]] | | [[Category: Wakatsuki, S.]] |
| [[Category: Super helix]] | | [[Category: Super helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:00:28 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:04:47 2008'' |
