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| {{STRUCTURE_2d7i| PDB=2d7i | SCENE= }} | | {{STRUCTURE_2d7i| PDB=2d7i | SCENE= }} |
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| '''Crsytal structure of pp-GalNAc-T10 with UDP, GalNAc and Mn2+'''
| | ===Crsytal structure of pp-GalNAc-T10 with UDP, GalNAc and Mn2+=== |
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| ==Overview==
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| Mucin-type O-glycans are important carbohydrate chains involved in differentiation and malignant transformation. Biosynthesis of the O-glycan is initiated by the transfer of N-acetylgalactosamine (GalNAc) which is catalyzed by UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases (pp-GalNAc-Ts). Here we present crystal structures of the pp-GalNAc-T10 isozyme, which has specificity for glycosylated peptides, in complex with the hydrolyzed donor substrate UDP-GalNAc and in complex with GalNAc-serine. A structural comparison with uncomplexed pp-GalNAc-T1 suggests that substantial conformational changes occur in two loops near the catalytic center upon donor substrate binding, and that a distinct interdomain arrangement between the catalytic and lectin domains forms a narrow cleft for acceptor substrates. The distance between the catalytic center and the carbohydrate-binding site on the lectin beta sub-domain influences the position of GalNAc glycosylation on GalNAc-glycosylated peptide substrates. A chimeric enzyme in which the two domains of pp-GalNAc-T10 are connected by a linker from pp-GalNAc-T1 acquires activity toward non-glycosylated acceptors, identifying a potential mechanism for generating the various acceptor specificities in different isozymes to produce a wide range of O-glycans.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16650853}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16650853 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16650853}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Beta trefoil]] | | [[Category: Beta trefoil]] |
| [[Category: Rossmann fold]] | | [[Category: Rossmann fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:48:32 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:16:49 2008'' |
