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| {{STRUCTURE_2c35| PDB=2c35 | SCENE= }} | | {{STRUCTURE_2c35| PDB=2c35 | SCENE= }} |
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| '''SUBUNITS RPB4 AND RPB7 OF HUMAN RNA POLYMERASE II'''
| | ===SUBUNITS RPB4 AND RPB7 OF HUMAN RNA POLYMERASE II=== |
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| ==Overview==
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| The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II (RNAP(II)) form a heterodimer that protrudes from the 10-subunit core of the enzyme. We have obtained crystals of the human Rpb4/Rpb7 heterodimer and determined the structure to 2.7 A resolution. The presence of putative RNA-binding domains on the Rpb7 subunit and the position of the heterodimer close to the RNA exit groove in the 12 subunit yeast polymerase complex strongly suggests a role for the heterodimer in binding and stabilizing the nascent RNA transcript. We have complemented the structural analysis with biochemical studies directed at dissecting the RNA-binding properties of the human Rpb4/Rpb7 complex and that of the homologous E/F complex from Methanocaldococcus jannaschii. A number of conserved, solvent-exposed residues in both the human Rpb7 subunit and the archaeal E subunit have been modified by site-directed mutagenesis and the mutants tested for RNA binding by performing electrophoretic mobility shift assays. These studies have identified an elongated surface region on the corresponding face of both subunit E and Rpb7 that is involved in RNA binding. The area spans the nucleic acid binding face of the OB fold, including the B4-B5 loop, but also extends towards the N-terminal domain. | | The line below this paragraph, {{ABSTRACT_PUBMED_16282592}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16282592 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16282592}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rna polymerase ii]] | | [[Category: Rna polymerase ii]] |
| [[Category: Transcription]] | | [[Category: Transcription]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:10:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:38:34 2008'' |
