379d: Difference between revisions

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[[Image:379d.~~gif~~|left|200px]]

[[Image:379d.png|left|200px]]

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~~'''~~THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE~~'''~~

===THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE===

~~==Overview==~~

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~~We have captured an 8.7 A conformational change that takes place in~~ the ~~cleavage site of~~ the ~~hammerhead ribozyme during self-cleavage~~, ~~using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that~~ is ~~poised to form~~ the ~~transition state geometry required for hammerhead RNA self-cleavage~~. ~~Use of a 5'~~-C-methylated ribose adjacent to the cleavage site permits this ordinarily transient conformational change to be kinetically trapped and observed crystallographically after initiating the hammerhead ribozyme reaction in the crystal. Cleavage of the corresponding unmodified hammerhead ribozyme in the crystal under otherwise identical conditions is faster than in solution, indicating that we have indeed trapped a catalytically relevant intermediate form of this RNA enzyme.

The line below this paragraph, {{ABSTRACT_PUBMED_9506521}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 9506521 is the PubMed ID number.

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==About this Structure==

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[[Category: Loop]]

[[Category: Rna hammerhead ribozyme]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4~~ 20:17:59 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:55:59 2008''

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-[[Image:379d.gif|left|200px]]
+{{Seed}}
+[[Image:379d.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_379d|  PDB=379d  |  SCENE=  }}
-'''THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE'''
+===THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE===
-==Overview==
+<!--
-We have captured an 8.7 A conformational change that takes place in the cleavage site of the hammerhead ribozyme during self-cleavage, using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that is poised to form the transition state geometry required for hammerhead RNA self-cleavage. Use of a 5'-C-methylated ribose adjacent to the cleavage site permits this ordinarily transient conformational change to be kinetically trapped and observed crystallographically after initiating the hammerhead ribozyme reaction in the crystal. Cleavage of the corresponding unmodified hammerhead ribozyme in the crystal under otherwise identical conditions is faster than in solution, indicating that we have indeed trapped a catalytically relevant intermediate form of this RNA enzyme.
+The line below this paragraph, {{ABSTRACT_PUBMED_9506521}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 9506521 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_9506521}}
 ==About this Structure==
@@ Line 30: / Line 34: @@
 [[Category: Loop]]
 [[Category: Rna hammerhead ribozyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:17:59 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:55:59 2008''