2b0j: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2b0j.gif|left|200px]]
{{Seed}}
[[Image:2b0j.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2b0j|  PDB=2b0j  |  SCENE=  }}  
{{STRUCTURE_2b0j|  PDB=2b0j  |  SCENE=  }}  


'''The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)'''
===The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)===




==Overview==
<!--  
The iron-sulphur cluster-free hydrogenase (Hmd, EC 1.12.98.2) from methanogenic archaea is a novel type of hydrogenase that tightly binds an iron-containing cofactor. The iron is coordinated by two CO molecules, one sulphur and a pyridone derivative, which is linked via a phosphodiester bond to a guanosine base. We report here on the crystal structure of the Hmd apoenzyme from Methanocaldococcus jannaschii at 1.75 A and from Methanopyrus kandleri at 2.4 A resolution. Homodimeric Hmd reveals a unique architecture composed of one central and two identical peripheral globular units. The central unit is composed of the intertwined C-terminal segments of both subunits, forming a novel intersubunit fold. The two peripheral units consist of the N-terminal domain of each subunit. The Rossmann fold-like structure of the N-terminal domain contains a mononucleotide-binding site, which could harbour the GMP moiety of the cofactor. Another binding site for the iron-containing cofactor is most probably Cys176, which is located at the bottom of a deep intersubunit cleft and which has been shown to be essential for enzyme activity. Adjacent to the iron of the cofactor modelled as a ligand to Cys176, an extended U-shaped extra electron density, interpreted as a polyethyleneglycol fragment, suggests a binding site for the substrate methenyltetrahydromethanopterin.
The line below this paragraph, {{ABSTRACT_PUBMED_16540118}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16540118 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16540118}}


==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Helix bundle]]
[[Category: Helix bundle]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:42:05 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:05:43 2008''

Revision as of 18:05, 27 July 2008

File:2b0j.png

Template:STRUCTURE 2b0j

The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)

Template:ABSTRACT PUBMED 16540118

About this Structure

2B0J is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

The crystal structure of the apoenzyme of the iron-sulphur cluster-free hydrogenase., Pilak O, Mamat B, Vogt S, Hagemeier CH, Thauer RK, Shima S, Vonrhein C, Warkentin E, Ermler U, J Mol Biol. 2006 May 5;358(3):798-809. Epub 2006 Mar 2. PMID:16540118

Page seeded by OCA on Sun Jul 27 21:05:43 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2b0j&oldid=667024"