2k10: Difference between revisions

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[[Image:2k10.jpg|left|200px]]
{{Seed}}
[[Image:2k10.png|left|200px]]


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{{STRUCTURE_2k10|  PDB=2k10  |  SCENE=  }}  
{{STRUCTURE_2k10|  PDB=2k10  |  SCENE=  }}  


'''Confirmational analysis of the broad-spectrum antibacterial peptide, rantuerin-2csa: identification of a full length helix-turn-helix motif'''
===Confirmational analysis of the broad-spectrum antibacterial peptide, rantuerin-2csa: identification of a full length helix-turn-helix motif===




==Overview==
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Design of clinically valuable antibacterial agents based upon naturally occurring peptides requires the use of spectroscopic methods, particularly NMR, to determine the three-dimensional structure of the native peptide so that analogues with improved therapeutic properties can be made. Ranatuerin-2CSa (GILSSFKGVAKGVAKDLAG KLLETLKCKITGC), first isolated from skin secretions of the Cascades frog, Rana cascadae, represents a promising candidate for drug development. The peptide shows potent growth inhibitory activity against Escherichia coli (MIC=5 muM) and Staphylococcus aureus (MIC=10 muM) but displays haemolytic activity against human erythrocytes (LC(50)=160 muM). The solution structure of ranatuerin-2CSa was investigated by proton NMR spectroscopy and molecular modelling. In aqueous solution, the peptide lacks secondary structure but, in a 2,2,2-trifluoroethanol (TFE-d(3))-H(2)O solvent mixture, the structure is characterised by a full length helix-turn-helix conformation between residues I(2)-L(21), L(22)-L(25) and K(26)-T(30) respectively. This structural information will facilitate the design of novel therapeutic agents based upon the ranatuerin-2CSa structure with improved antimicrobial potencies but decreased cytolytic activities against mammalian cells.
The line below this paragraph, {{ABSTRACT_PUBMED_18387372}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_18387372}}


==About this Structure==
==About this Structure==
2K10 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K10 OCA].  
2K10 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K10 OCA].  


==Reference==
==Reference==
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[[Category: Molecular modelling]]
[[Category: Molecular modelling]]
[[Category: Nmr]]
[[Category: Nmr]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun  4 09:57:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:08:37 2008''

Revision as of 18:08, 27 July 2008

File:2k10.png

Template:STRUCTURE 2k10

Confirmational analysis of the broad-spectrum antibacterial peptide, rantuerin-2csa: identification of a full length helix-turn-helix motif

Template:ABSTRACT PUBMED 18387372

About this Structure

2K10 is a Single protein structure. Full experimental information is available from OCA.

Reference

Conformational analysis of the broad-spectrum antibacterial peptide, ranatuerin-2CSa: Identification of a full length helix-turn-helix motif., Subasinghage AP, Conlon JM, Hewage CM, Biochim Biophys Acta. 2008 Jun;1784(6):924-9. Epub 2008 Mar 14. PMID:18387372

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