1rxq: Difference between revisions

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[[Image:1rxq.gif|left|200px]]
{{Seed}}
[[Image:1rxq.png|left|200px]]


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{{STRUCTURE_1rxq|  PDB=1rxq  |  SCENE=  }}  
{{STRUCTURE_1rxq|  PDB=1rxq  |  SCENE=  }}  


'''YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology'''
===YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology===




==Overview==
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YfiT, a 19-kDa polypeptide from Bacillus subtilis, belongs to a small sequence family with members predominantly from Gram positive bacteria. We have determined the crystal structure of YfiT in complex with Ni(2+) to a resolution of 1.7 A. YfiT exists as a dimer and binds Ni(2+) in a 1:1 stoichiometry. The protein has an unusual four-helix bundle topology and coordinates Ni(2+) in an octahedral geometry with three conserved histidines and three waters. Although there is no similarity in their overall structures, the coordination geometry of the metal and the residues that constitute the putative active site in YfiT are similar to those of metalloproteases such as thermolysin. Our structural analyses suggest that YfiT might function as a metal-dependent hydrolase.
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{{ABSTRACT_PUBMED_15581359}}


==About this Structure==
==About this Structure==
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[[Category: Psi]]
[[Category: Psi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:02:00 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:27:48 2008''

Revision as of 18:27, 27 July 2008

File:1rxq.png

Template:STRUCTURE 1rxq

YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology

Template:ABSTRACT PUBMED 15581359

About this Structure

1RXQ is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology., Rajan SS, Yang X, Shuvalova L, Collart F, Anderson WF, Biochemistry. 2004 Dec 14;43(49):15472-9. PMID:15581359

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