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| {{STRUCTURE_2uvi| PDB=2uvi | SCENE= }} | | {{STRUCTURE_2uvi| PDB=2uvi | SCENE= }} |
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| '''STRUCTURE OF A PERIPLASMIC OLIGOGALACTURONIDE BINDING PROTEIN FROM YERSINIA ENTEROCOLITICA IN COMPLEX WITH 4,5-UNSATURATED DIGALACTURONIC ACID'''
| | ===STRUCTURE OF A PERIPLASMIC OLIGOGALACTURONIDE BINDING PROTEIN FROM YERSINIA ENTEROCOLITICA IN COMPLEX WITH 4,5-UNSATURATED DIGALACTURONIC ACID=== |
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| ==Overview==
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| The process of pectin depolymerization by pectate lyases and glycoside hydrolases produced by pectinolytic organisms, particularly the phytopathogens from the genus Erwinia, is reasonably well understood. Indeed each extracellular and intracellular catabolic stage has been identified using either genetic, bioinformatic or biochemical approaches. Nevertheless, the molecular details of many of these stages remain unknown. In particular, the mechanism and ligand binding profiles for the transport of pectin degradation products between cellular compartments remain entirely uninvestigated. Here we present the structure of TogB, a 45.7 kDa periplasmic binding protein from Yersinia enterocolitica. This protein is a component of the TogMNAB ABC transporter involved in the periplasmic transport of oligogalacturonides. In addition to the unliganded complex (at 2.2 A), we have also determined the structures of TogB in complex with digalacturonic acid (at 2.2 A), trigalacturonic acid (at 1.8 A) and 4,5-unsaturated digalacutronic acid (at 2.3 A). The molecular determinants of oligogalacturonide binding include a novel salt-bridge between the non-reducing sugar uronate group, selectivity for the unsaturated ligand, and the overall sugar configuration. Complementing this are UV difference and isothermal titration calorimetry experiments that highlight the thermodynamic basis of ligand specificity. The ligand binding profiles of the TogMNAB transporter complex nicely complement pectate lyase-mediated pectin degradation, which is a significant component of pectin depolymerization reactions. | | The line below this paragraph, {{ABSTRACT_PUBMED_17451747}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17451747 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17451747}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Togb]] | | [[Category: Togb]] |
| [[Category: Yersinia enterocolitica]] | | [[Category: Yersinia enterocolitica]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:51:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:29:13 2008'' |
