2e4i: Difference between revisions

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[[Image:2e4i.gif|left|200px]]
{{Seed}}
[[Image:2e4i.png|left|200px]]


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{{STRUCTURE_2e4i|  PDB=2e4i  |  SCENE=  }}  
{{STRUCTURE_2e4i|  PDB=2e4i  |  SCENE=  }}  


'''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines'''
===Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines===




==Overview==
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The structure of human telomeric DNA is controversial; it depends upon the sequence contexts and the methodologies used to determine it. The solution structure in the presence of K(+) is particularly interesting, but the structure is yet to be elucidated, due to possible conformational heterogeneity. Here, a unique strategy is applied to stabilize one such structure in a K(+) solution by substituting guanosines with 8-bromoguanosines at proper positions. The resulting spectra are cleaner and led to determination of the structure at a high atomic resolution. This demonstrates that the application of 8-bromoguanosine is a powerful tool to overcome the difficulty of nucleic acid structure determination arising from conformational heterogeneity. The obtained structure is a mixed-parallel/antiparallel quadruplex. The structure of telomeric DNA was recently reported in another study, in which stabilization was brought about by mutation and resultant additional interactions [Luu KN, Phan AT, Kuryavyi V, Lacroix L &amp; Patel DJ (2006) Structure of the human telomere in K(+) solution: an intramolecular (3+1) G-quadruplex scaffold. J Am Chem Soc 128, 9963-9970]. The structure of the guanine tracts was similar between the two. However, a difference was seen for loops connecting guanine tracts, which may play a role in the higher order arrangement of telomeres. Our structure can be utilized to design a small molecule which stabilizes the quadruplex. This type of molecule is supposed to inhibit a telomerase and thus is expected to be a candidate anticancer drug.
The line below this paragraph, {{ABSTRACT_PUBMED_17561958}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 17561958 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17561958}}


==About this Structure==
==About this Structure==
2E4I is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4I OCA].  
2E4I is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4I OCA].  


==Reference==
==Reference==
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[[Category: Structure]]
[[Category: Structure]]
[[Category: Telomere]]
[[Category: Telomere]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:54:11 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:43:24 2008''

Revision as of 19:43, 27 July 2008

File:2e4i.png

Template:STRUCTURE 2e4i

Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines

Template:ABSTRACT PUBMED 17561958

About this Structure

2E4I is a Single protein structure. Full experimental information is available from OCA.

Reference

Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution., Matsugami A, Xu Y, Noguchi Y, Sugiyama H, Katahira M, FEBS J. 2007 Jul;274(14):3545-56. Epub 2007 Jun 11. PMID:17561958

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