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| [[Image:1wql.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1wql| PDB=1wql | SCENE= }} | | {{STRUCTURE_1wql| PDB=1wql | SCENE= }} |
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| '''Cumene dioxygenase (cumA1A2) from Pseudomonas fluorescens IP01'''
| | ===Cumene dioxygenase (cumA1A2) from Pseudomonas fluorescens IP01=== |
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| ==Overview==
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| The crystal structure of the terminal component of the cumene dioxygenase multicomponent enzyme system of Pseudomonas fluorescens IP01 (CumDO) was determined at a resolution of 2.2 A by means of molecular replacement by using the crystal structure of the terminal oxygenase component of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4 (NphDO). The ligation of the two catalytic centers of CumDO (i.e., the nonheme iron and Rieske [2Fe-2S] centers) and the bridging between them in neighboring catalytic subunits by hydrogen bonds through a single amino acid residue, Asp231, are similar to those of NphDO. An unidentified external ligand, possibly dioxygen, was bound at the active site nonheme iron. The entrance to the active site of CumDO is different from the entrance to the active site of NphDO, as the two loops forming the lid exhibit great deviation. On the basis of the complex structure of NphDO, a biphenyl substrate was modeled in the substrate-binding pocket of CumDO. The residues surrounding the modeled biphenyl molecule include residues that have already been shown to be important for its substrate specificity by a number of engineering studies of biphenyl dioxygenases. | | The line below this paragraph, {{ABSTRACT_PUBMED_15774891}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15774891 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15774891}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Polychlorinated biphenyl degradation]] | | [[Category: Polychlorinated biphenyl degradation]] |
| [[Category: Rieske non-heme iron dioxygenase]] | | [[Category: Rieske non-heme iron dioxygenase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:01:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:01:24 2008'' |
