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| {{STRUCTURE_1sz0| PDB=1sz0 | SCENE= }} | | {{STRUCTURE_1sz0| PDB=1sz0 | SCENE= }} |
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| '''N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate'''
| | ===N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate=== |
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| ==Overview==
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| The 300-kDa cation-independent mannose 6-phosphate receptor (CI-MPR) plays a critical role in the trafficking of newly synthesized mannose 6-phosphate-containing acid hydrolases to the lysosome. The receptor contains two high affinity carbohydrate recognition sites within its 15-domain extracytoplasmic region, with essential residues for carbohydrate recognition located in domain 3 and domain 9. Previous studies have shown that these two sites are distinct with respect to carbohydrate specificity. In addition, expression of truncated forms of the CI-MPR demonstrated that domain 9 can be expressed as an isolated domain, retaining high affinity (Kd approximately 1 nm) carbohydrate binding, whereas expression of domain 3 alone resulted in a protein capable of only low affinity binding (Kd approximately 1 microm) toward a lysosomal enzyme. In the current report the crystal structure of the N-terminal 432 residues of the CI-MPR, encompassing domains 1-3, was solved in the presence of bound mannose 6-phosphate. The structure reveals the unique architecture of this carbohydrate binding pocket and provides insight into the ability of this site to recognize a variety of mannose-containing sugars. | | The line below this paragraph, {{ABSTRACT_PUBMED_15169779}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15169779 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15169779}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Mannose 6-phosphate]] | | [[Category: Mannose 6-phosphate]] |
| [[Category: Receptor]] | | [[Category: Receptor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:17:51 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:33:22 2008'' |
