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[[Image:2dyo.jpg|left|200px]]
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[[Image:2dyo.png|left|200px]]


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{{STRUCTURE_2dyo|  PDB=2dyo  |  SCENE=  }}  
{{STRUCTURE_2dyo|  PDB=2dyo  |  SCENE=  }}  


'''The crystal structure of Saccharomyces cerevisiae Atg5- Atg16(1-57) complex'''
===The crystal structure of Saccharomyces cerevisiae Atg5- Atg16(1-57) complex===




==Overview==
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Atg5 is covalently modified with a ubiquitin-like modifier, Atg12, and the Atg12-Atg5 conjugate further forms a complex with the multimeric protein Atg16. The Atg12-Atg5.Atg16 multimeric complex plays an essential role in autophagy, the bulk degradation system conserved in all eukaryotes. We have reported here the crystal structure of Atg5 complexed with the N-terminal region of Atg16 at 1.97A resolution. Atg5 comprises two ubiquitin-like domains that flank a helix-rich domain. The N-terminal region of Atg16 has a helical structure and is bound to the groove formed by these three domains. In vitro analysis showed that Arg-35 and Phe-46 of Atg16 are crucial for the interaction. Atg16, with a mutation at these residues, failed to localize to the pre-autophagosomal structure and could not restore autophagy in Atg16-deficient yeast strains. Furthermore, these Atg16 mutants could not restore a severe reduction in the formation of the Atg8-phosphatidylethanolamine conjugate, another essential factor for autophagy, in Atg16-deficient strains under starvation conditions. These results taken together suggest that the direct interaction between Atg5 and Atg16 is crucial to the performance of their roles in autophagy.
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{{ABSTRACT_PUBMED_17192262}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of Atg5.Atg16, a complex essential for autophagy., Matsushita M, Suzuki NN, Obara K, Fujioka Y, Ohsumi Y, Inagaki F, J Biol Chem. 2007 Mar 2;282(9):6763-72. Epub 2006 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17192262 17192262]
Structure of Atg5.Atg16, a complex essential for autophagy., Matsushita M, Suzuki NN, Obara K, Fujioka Y, Ohsumi Y, Inagaki F, J Biol Chem. 2007 Mar 2;282(9):6763-72. Epub 2006 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17192262 17192262]
Expression, purification and crystallization of the Atg5-Atg16 complex essential for autophagy., Matsushita M, Suzuki NN, Fujioka Y, Ohsumi Y, Inagaki F, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):1021-3. Epub 2006 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17012802 17012802]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Herix-bundle]]
[[Category: Herix-bundle]]
[[Category: Ubiquitin-fold]]
[[Category: Ubiquitin-fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:37:46 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:44:03 2008''

Revision as of 20:44, 27 July 2008

File:2dyo.png

Template:STRUCTURE 2dyo

The crystal structure of Saccharomyces cerevisiae Atg5- Atg16(1-57) complex

Template:ABSTRACT PUBMED 17192262

About this Structure

2DYO is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of Atg5.Atg16, a complex essential for autophagy., Matsushita M, Suzuki NN, Obara K, Fujioka Y, Ohsumi Y, Inagaki F, J Biol Chem. 2007 Mar 2;282(9):6763-72. Epub 2006 Dec 27. PMID:17192262

Expression, purification and crystallization of the Atg5-Atg16 complex essential for autophagy., Matsushita M, Suzuki NN, Fujioka Y, Ohsumi Y, Inagaki F, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt, 10):1021-3. Epub 2006 Sep 30. PMID:17012802

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