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| [[Image:1yvu.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1yvu| PDB=1yvu | SCENE= }} | | {{STRUCTURE_1yvu| PDB=1yvu | SCENE= }} |
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| '''Crystal structure of A. aeolicus Argonaute'''
| | ===Crystal structure of A. aeolicus Argonaute=== |
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| ==Overview==
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| Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16061186}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16061186 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16061186}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Zhadina, M.]] | | [[Category: Zhadina, M.]] |
| [[Category: Rnase h fold]] | | [[Category: Rnase h fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:51:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:51:35 2008'' |
