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| {{STRUCTURE_1ynt| PDB=1ynt | SCENE= }} | | {{STRUCTURE_1ynt| PDB=1ynt | SCENE= }} |
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| '''Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody'''
| | ===Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody=== |
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| ==Overview==
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| Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16242717}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16242717 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16242717}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Recombinant sag1]] | | [[Category: Recombinant sag1]] |
| [[Category: Toxoplasma gondii]] | | [[Category: Toxoplasma gondii]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:33:29 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:56:30 2008'' |
