2hfh: Difference between revisions

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[[Image:2hfh.jpg|left|200px]]
{{Seed}}
[[Image:2hfh.png|left|200px]]


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{{STRUCTURE_2hfh|  PDB=2hfh  |  SCENE=  }}  
{{STRUCTURE_2hfh|  PDB=2hfh  |  SCENE=  }}  


'''THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES'''
===THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES===




==Overview==
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The hepatocyte nuclear factor 3 (HNF-3)/fork head (fkh) family contains a large number of transcription factors and folds into a winged helix motif. Despite having almost invariable amino acid sequences in their principal DNA-binding helices, HNF-3/fkh proteins show a wide diversity of sequence-specific binding. Previous studies of chimeric HNF-3/fkh proteins demonstrated that the binding specificity is primarily influenced by a region directly adjacent to the binding helix. We report our findings of an NMR structural study performed on an HNF-3/fkh family member (Genesis, formerly HFH-2) and compare it to that of another family member (HNF-3gamma) complexed to DNA and determined by X-ray crystallography. It is found that in comparison to HNF-3gamma, Genesis contains an extra small helix directly prior to the N terminus of the primary DNA contact helix. Due to the insertion of this helix, a shorter and slightly re-positioned primary DNA contact helix is observed, which we believe leads to the DNA-binding specificity differences among family members.
The line below this paragraph, {{ABSTRACT_PUBMED_9571051}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9571051 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9571051}}


==About this Structure==
==About this Structure==
2HFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFH OCA].  
2HFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFH OCA].  


==Reference==
==Reference==
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[[Category: Hnf-3 homologue]]
[[Category: Hnf-3 homologue]]
[[Category: Winged helix protein]]
[[Category: Winged helix protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:13:47 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:13:16 2008''

Revision as of 21:13, 27 July 2008

File:2hfh.png

Template:STRUCTURE 2hfh

THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES

Template:ABSTRACT PUBMED 9571051

About this Structure

2HFH is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins., Marsden I, Jin C, Liao X, J Mol Biol. 1998 May 1;278(2):293-9. PMID:9571051

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