2q97: Difference between revisions

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[[Image:2q97.gif|left|200px]]
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[[Image:2q97.png|left|200px]]


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{{STRUCTURE_2q97|  PDB=2q97  |  SCENE=  }}  
{{STRUCTURE_2q97|  PDB=2q97  |  SCENE=  }}  


'''Complex of mammalian actin with toxofilin from toxoplasma gondii'''
===Complex of mammalian actin with toxofilin from toxoplasma gondii===




==Overview==
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Many human pathogens exploit the actin cytoskeleton during infection, including Toxoplasma gondii, an apicomplexan parasite related to Plasmodium, the agent of malaria. One of the most abundantly expressed proteins of T. gondii is toxofilin, a monomeric actin-binding protein (ABP) involved in invasion. Toxofilin is found in rhoptry and presents an N-terminal signal sequence, consistent with its being secreted during invasion. We report the structure of toxofilin amino acids 69-196 in complex with the host mammalian actin. Toxofilin presents an extended conformation and interacts with an antiparallel actin dimer, in which one of the actins is related by crystal symmetry. Consistent with this observation, analytical ultracentrifugation analysis shows that toxofilin binds two actins in solution. Toxofilin folds into five consecutive helices, which form three relatively independent actin-binding sites. Helices 1 and 2 bind the symmetry-related actin molecule and cover its nucleotide-binding cleft. Helices 3-5 bind the other actin and constitute the primary actin-binding region. Helix 3 interacts in the cleft between subdomains 1 and 3, a common binding site for most ABPs. Helices 4 and 5 wrap around actin subdomain 4, and residue Gln-134 of helix 4 makes a hydrogen-bonding contact with the nucleotide in actin, both of which are unique features among ABPs. Toxofilin dramatically inhibits nucleotide exchange on two actin molecules simultaneously. This effect is linked to the formation of the antiparallel actin dimer because a construct lacking helices 1 and 2 binds only one actin and inhibits nucleotide exchange less potently.
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{{ABSTRACT_PUBMED_17911258}}


==About this Structure==
==About this Structure==
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[[Category: Structural protein]]
[[Category: Structural protein]]
[[Category: Structural protein/cell invasion complex]]
[[Category: Structural protein/cell invasion complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:33:41 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:16:35 2008''

Revision as of 21:16, 27 July 2008

File:2q97.png

Template:STRUCTURE 2q97

Complex of mammalian actin with toxofilin from toxoplasma gondii

Template:ABSTRACT PUBMED 17911258

About this Structure

2Q97 is a Protein complex structure of sequences from Oryctolagus cuniculus and Toxoplasma gondii. Full crystallographic information is available from OCA.

Reference

Toxofilin from Toxoplasma gondii forms a ternary complex with an antiparallel actin dimer., Lee SH, Hayes DB, Rebowski G, Tardieux I, Dominguez R, Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16122-7. Epub 2007 Oct 2. PMID:17911258

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