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| [[Image:1mz9.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1mz9.png|left|200px]] |
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| {{STRUCTURE_1mz9| PDB=1mz9 | SCENE= }} | | {{STRUCTURE_1mz9| PDB=1mz9 | SCENE= }} |
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| '''Storage function of COMP:the crystal structure of the coiled-coil domain in complex with vitamin D3'''
| | ===Storage function of COMP:the crystal structure of the coiled-coil domain in complex with vitamin D3=== |
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| ==Overview==
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| The five-stranded coiled-coil domain of cartilage oligomeric matrix protein (COMPcc) forms a continuous axial pore with binding capacities for hydrophobic compounds, including prominent cell signalling molecules. Here, we report the X-ray structure of the COMPcc domain in complex with vitamin D(3) at 1.7 A resolution. The COMPcc pentamer harbours two molecules of the steroid hormone precursor in a planar s-trans conformation of the conjugated triene, with the aliphatic tails lying along the molecule axis. A hydrophilic ring of five Gln54 side chains divides the channel into two hydrophobic compartments in which the bound vitamin D(3) pair is fixed in a head-to-head orientation. Vitamin D(3) binding induces a volumetric increase of the cavities of approximately 30% while the main chain distances of the pentamer are retained. This adaptation to the bulky ring systems of the ligands is accomplished by a rotamer re-orientation of beta-branched side chains that form the knobs into holes of the coiled-coil structure. Compared with binding of vitamin D and retinoic acid by their classical receptors, COMP exerts a distinct mechanism of interaction mainly defined by the pattern of hydrophobic core residues. | | The line below this paragraph, {{ABSTRACT_PUBMED_12426368}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12426368 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12426368}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Stetefeld, J.]] | | [[Category: Stetefeld, J.]] |
| [[Category: Pentameric coiled-coil domain]] | | [[Category: Pentameric coiled-coil domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:53:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:21:55 2008'' |
