1y29: Difference between revisions

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[[Image:1y29.gif|left|200px]]
{{Seed}}
[[Image:1y29.png|left|200px]]


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{{STRUCTURE_1y29|  PDB=1y29  |  SCENE=  }}  
{{STRUCTURE_1y29|  PDB=1y29  |  SCENE=  }}  


'''Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR'''
===Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR===




==Overview==
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Huwentoxin-X (HWTX-X) is a novel peptide toxin, purified from the venom of the spider Ornithoctonus huwena. It comprises 28 amino acid residues including six cysteine residues as disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Its cDNA, determined by rapid amplification of 3' and 5' cDNA ends, encodes a 65-residue prepropeptide. HWTX-X shares low sequence homology with omega-conotoxins GVIA and MVIIA, two well known blockers of N-type Ca2+ channels. Nevertheless, whole cell studies indicate that it can block N-type Ca2+ channels in rat dorsal root ganglion cells (IC50 40 nm) and the blockage by HWTX-X is completely reversible. The rank order of specificity for N-type Ca2+ channels is GVIA approximately HWTX-X &gt; MVIIA. In contrast to GVIA and MVIIA, HWTX-X had no detectable effect on the twitch response of rat vas deferens to low frequency electrical stimulation, indicating that HWTX-X has different selectivity for isoforms of N-type Ca2+ channels, compared with GVIA or MVIIA. A comparison of the structures of HWTX-X and GVIA reveals that they not only adopt a common structural motif (inhibitor cystine knot), but also have a similar functional motif, a binding surface formed by the critical residue Tyr, and several basic residues. However, the dissimilarities of their binding surfaces provide some insights into their different selectivities for isoforms of N-type Ca2+ channels.
The line below this paragraph, {{ABSTRACT_PUBMED_16439354}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_16439354}}


==About this Structure==
==About this Structure==
1Y29 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA].  
1Y29 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA].  


==Reference==
==Reference==
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[[Category: Ick]]
[[Category: Ick]]
[[Category: Knottin]]
[[Category: Knottin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:47:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:29:57 2008''

Revision as of 21:29, 27 July 2008

File:1y29.png

Template:STRUCTURE 1y29

Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR

Template:ABSTRACT PUBMED 16439354

About this Structure

1Y29 is a Single protein structure. Full experimental information is available from OCA.

Reference

Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena., Liu Z, Dai J, Dai L, Deng M, Hu Z, Hu W, Liang S, J Biol Chem. 2006 Mar 31;281(13):8628-35. Epub 2006 Jan 26. PMID:16439354

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