3buc: Difference between revisions

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[[Image:3buc.~~jpg~~|left|200px]]

[[Image:3buc.png|left|200px]]

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~~'''~~X-ray structure of human ABH2 bound to dsDNA with Mn(II) and 2KG~~'''~~

===X-ray structure of human ABH2 bound to dsDNA with Mn(II) and 2KG===

~~==Overview==~~

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Escherichia coli AlkB and its human homologues ABH2 and ABH3 repair DNA/RNA base lesions by using a direct oxidative dealkylation mechanism. ABH2 has the primary role of guarding mammalian genomes against 1-meA damage by repairing this ~~lesion in double-stranded DNA (dsDNA)~~, ~~whereas AlkB and ABH3 preferentially repair single-stranded DNA (ssDNA) lesions and can repair damaged bases in RNA. Here we show~~ the ~~first crystal structures of AlkB-dsDNA and ABH2-dsDNA complexes, stabilized by a chemical cross-linking strategy. This study reveals that AlkB uses an unprecedented base-flipping mechanism~~ to ~~access~~ the ~~damaged base~~: ~~it squeezes together the two bases flanking the flipped-out one to maintain the base stack, explaining the preference of AlkB for repairing ssDNA lesions over dsDNA ones~~. ~~In addition~~, the ~~first crystal structure of ABH2, presented here, provides a structural basis for designing inhibitors of this human DNA repair protein~~.

The line below this paragraph, {{ABSTRACT_PUBMED_18432238}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 18432238 is the PubMed ID number.

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==About this Structure==

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[[Category: Oxidoreductase/dna complex]]

[[Category: Protein/dna interaction]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 11 10~~:57:40 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:39:06 2008''

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-[[Image:3buc.jpg|left|200px]]
+{{Seed}}
+[[Image:3buc.png|left|200px]]
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 {{STRUCTURE_3buc|  PDB=3buc  |  SCENE=  }}
-'''X-ray structure of human ABH2 bound to dsDNA with Mn(II) and 2KG'''
+===X-ray structure of human ABH2 bound to dsDNA with Mn(II) and 2KG===
-==Overview==
+<!--
-Escherichia coli AlkB and its human homologues ABH2 and ABH3 repair DNA/RNA base lesions by using a direct oxidative dealkylation mechanism. ABH2 has the primary role of guarding mammalian genomes against 1-meA damage by repairing this lesion in double-stranded DNA (dsDNA), whereas AlkB and ABH3 preferentially repair single-stranded DNA (ssDNA) lesions and can repair damaged bases in RNA. Here we show the first crystal structures of AlkB-dsDNA and ABH2-dsDNA complexes, stabilized by a chemical cross-linking strategy. This study reveals that AlkB uses an unprecedented base-flipping mechanism to access the damaged base: it squeezes together the two bases flanking the flipped-out one to maintain the base stack, explaining the preference of AlkB for repairing ssDNA lesions over dsDNA ones. In addition, the first crystal structure of ABH2, presented here, provides a structural basis for designing inhibitors of this human DNA repair protein.
+The line below this paragraph, {{ABSTRACT_PUBMED_18432238}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18432238 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18432238}}
 ==About this Structure==
@@ Line 35: / Line 39: @@
 [[Category: Oxidoreductase/dna complex]]
 [[Category: Protein/dna interaction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:57:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:39:06 2008''