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| [[Image:1uzv.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1uzv| PDB=1uzv | SCENE= }} | | {{STRUCTURE_1uzv| PDB=1uzv | SCENE= }} |
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| '''HIGH AFFINITY FUCOSE BINDING OF PSEUDOMONAS AERUGINOSA LECTIN II: 1.0 A CRYSTAL STRUCTURE OF THE COMPLEX'''
| | ===HIGH AFFINITY FUCOSE BINDING OF PSEUDOMONAS AERUGINOSA LECTIN II: 1.0 A CRYSTAL STRUCTURE OF THE COMPLEX=== |
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| ==Overview==
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| PA-IIL is a fucose-binding lectin from Pseudomonas aeruginosa that is closely related to the virulence factors of the bacterium. Previous structural studies have revealed a new carbohydrate-binding mode with direct involvement of two calcium ions (Mitchell E, Houles C, Sudakevitz D, Wimmerova M, Gautier C, Perez S, Wu AM, Gilboa-Garber N, Imberty A. Structural basis for selective recognition of oligosaccharides from cystic fibrosis patients by the lectin PA-IIL of Pseudomonas aeruginosa. Nat Struct Biol 2002;9:918-921). A combination of thermodynamic, structural, and computational methods has been used to study the basis of the high affinity for the monosaccharide ligand. A titration microcalorimetry study indicated that the high affinity is enthalpy driven. The crystal structure of the tetrameric PA-IIL in complex with fucose and calcium was refined to 1.0 A resolution and, in combination with modeling, allowed a proposal to be made for the hydrogen-bond network in the binding site. Calculations of partial charges using ab initio computational chemistry methods indicated that extensive delocalization of charges between the calcium ions, the side chains of the protein-binding site and the carbohydrate ligand is responsible for the high enthalpy of binding and therefore for the unusually high affinity observed for this unique mode of carbohydrate recognition.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15573375}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15573375 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15573375}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Fucose]] | | [[Category: Fucose]] |
| [[Category: Lectin]] | | [[Category: Lectin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:54:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:04:24 2008'' |
