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| [[Image:2q17.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2q17| PDB=2q17 | SCENE= }} | | {{STRUCTURE_2q17| PDB=2q17 | SCENE= }} |
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| '''Formylglycine Generating Enzyme from Streptomyces coelicolor'''
| | ===Formylglycine Generating Enzyme from Streptomyces coelicolor=== |
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| ==Overview==
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| Type I sulfatases require an unusual co- or post-translational modification for their activity in hydrolyzing sulfate esters. In eukaryotic sulfatases, an active site cysteine residue is oxidized to the aldehyde-containing Ca-formylglycine (FGly) residue by the formylglycine generating enzyme (FGE). The machinery responsible for sulfatase activation is poorly understood in prokaryotes. Here we describe the identification of a prokaryotic FGE from M. tuberculosis. In addition, we solved the crystal structure of the Streptomyces coelicolor FGE homolog to 2.1 A resolution. The prokaryotic homolog exhibits remarkable structural similarity to human FGE, including the position of catalytic cysteine residues. Both biochemical and structural data indicate the presence of an oxidized cysteine modification in the active site, which may be relevant to catalysis. In addition, we generated a mutant M. tuberculosis strain lacking FGE. Although global sulfatase activity was reduced in the mutant, a significant amount of residual sulfatase activity suggests the presence of FGE-independent sulfatases in this organism.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18390551}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18390551 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18390551}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sulfatase]] | | [[Category: Sulfatase]] |
| [[Category: Unknown function]] | | [[Category: Unknown function]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:50:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:07:12 2008'' |
