|
|
| Line 1: |
Line 1: |
| [[Image:2j0x.gif|left|200px]] | | {{Seed}} |
| | [[Image:2j0x.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2j0x| PDB=2j0x | SCENE= }} | | {{STRUCTURE_2j0x| PDB=2j0x | SCENE= }} |
|
| |
|
| '''CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)'''
| | ===CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Aspartokinase III (AKIII) from Escherichia coli catalyzes an initial commitment step of the aspartate pathway, giving biosynthesis of certain amino acids including lysine. We report crystal structures of AKIII in the inactive T-state with bound feedback allosteric inhibitor lysine and in the R-state with aspartate and ADP. The structures reveal an unusual configuration for the regulatory ACT domains, in which ACT2 is inserted into ACT1 rather than the expected tandem repeat. Comparison of R- and T-state AKIII indicates that binding of lysine to the regulatory ACT1 domain in R-state AKIII instigates a series of changes that release a "latch", the beta15-alphaK loop, from the catalytic domain, which in turn undergoes large rotational rearrangements, promoting tetramer formation and completion of the transition to the T-state. Lysine-induced allosteric transition in AKIII involves both destabilizing the R-state and stabilizing the T-state tetramer. Rearrangement of the catalytic domain blocks the ATP-binding site, which is therefore the structural basis for allosteric inhibition of AKIII by lysine.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16905770}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16905770 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16905770}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 39: |
Line 43: |
| [[Category: Lysine biosynthesis]] | | [[Category: Lysine biosynthesis]] |
| [[Category: Transferase]] | | [[Category: Transferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:11:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:21:24 2008'' |
