2br6: Difference between revisions

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[[Image:2br6.gif|left|200px]]
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{{STRUCTURE_2br6|  PDB=2br6  |  SCENE=  }}  
{{STRUCTURE_2br6|  PDB=2br6  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF QUORUM-QUENCHING N-ACYL HOMOSERINE LACTONE LACTONASE'''
===CRYSTAL STRUCTURE OF QUORUM-QUENCHING N-ACYL HOMOSERINE LACTONE LACTONASE===




==Overview==
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In many Gram-negative bacteria, including a number of pathogens such as Pseudomonas aeruginosa and Erwinia carotovora, virulence factor production and biofilm formation are linked to the quorum-sensing systems that use diffusible N-acyl-L-homoserine lactones (AHLs) as intercellular messenger molecules. A number of organisms also contain genes coding for lactonases that hydrolyze AHLs into inactive products, thereby blocking the quorum-sensing systems. Consequently, these enzymes attract intense interest for the development of antiinfection therapies. However, the catalytic mechanism of AHL-lactonase is poorly understood and subject to controversy. We here report a 2.0-angstroms resolution structure of the AHL-lactonase from Bacillus thuringiensis and a 1.7-angstroms crystal structure of its complex with L-homoserine lactone. Despite limited sequence similarity, the enzyme shows remarkable structural similarities to glyoxalase II and RNase Z proteins, members of the metallo-beta-lactamase superfamily. We present experimental evidence that AHL-lactonase is a metalloenzyme containing two zinc ions involved in catalysis, and we propose a catalytic mechanism for bacterial metallo-AHL-lactonases.
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==About this Structure==
==About this Structure==
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[[Category: Quorum sensing]]
[[Category: Quorum sensing]]
[[Category: Quorum-quenching enzyme]]
[[Category: Quorum-quenching enzyme]]
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Revision as of 23:41, 27 July 2008

File:2br6.png

Template:STRUCTURE 2br6

CRYSTAL STRUCTURE OF QUORUM-QUENCHING N-ACYL HOMOSERINE LACTONE LACTONASE

Template:ABSTRACT PUBMED 16314577

About this Structure

2BR6 is a Single protein structure of sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA.

Reference

The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase., Kim MH, Choi WC, Kang HO, Lee JS, Kang BS, Kim KJ, Derewenda ZS, Oh TK, Lee CH, Lee JK, Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17606-11. Epub 2005 Nov 28. PMID:16314577

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