2hji: Difference between revisions

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[[Image:2hji.gif|left|200px]]
{{Seed}}
[[Image:2hji.png|left|200px]]


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{{STRUCTURE_2hji|  PDB=2hji  |  SCENE=  }}  
{{STRUCTURE_2hji|  PDB=2hji  |  SCENE=  }}  


'''Structural model for the Fe-containing isoform of acireductone dioxygenase'''
===Structural model for the Fe-containing isoform of acireductone dioxygenase===




==Overview==
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Acireductone dioxygenase (ARD) catalyzes different reactions between O2 and 1,2-dihydroxy-3-oxo-5-(methylthio)pent-1-ene (acireductone) depending upon the metal bound in the active site. Ni2+ -ARD cleaves acireductone to formate, CO and methylthiopropionate. If Fe2+ is bound (ARD'), the same substrates yield methylthioketobutyrate and formate. The two forms differ in structure, and are chromatographically separable. Paramagnetism of Fe2+ renders the active site of ARD' inaccessible to standard NMR methods. The structure of ARD' has been determined using Fe2+ binding parameters determined by X-ray absorption spectroscopy and NMR restraints from H98S ARD, a metal-free diamagnetic protein that is isostructural with ARD'. ARD' retains the beta-sandwich fold of ARD, but a structural entropy switch increases order at one end of a two-helix system that bisects the beta-sandwich and decreases order at the other upon interconversion of ARD and ARD', causing loss of the C-terminal helix in ARD' and rearrangements of residues involved in substrate orientation in the active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 16989860 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16989860}}


==About this Structure==
==About this Structure==
2HJI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_oxytoca Klebsiella oxytoca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJI OCA].  
2HJI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_oxytoca Klebsiella oxytoca]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJI OCA].  


==Reference==
==Reference==
One protein, two enzymes revisited: a structural entropy switch interconverts the two isoforms of acireductone dioxygenase., Ju T, Goldsmith RB, Chai SC, Maroney MJ, Pochapsky SS, Pochapsky TC, J Mol Biol. 2006 Nov 3;363(4):823-34. Epub 2006 Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16989860 16989860]
One protein, two enzymes revisited: a structural entropy switch interconverts the two isoforms of acireductone dioxygenase., Ju T, Goldsmith RB, Chai SC, Maroney MJ, Pochapsky SS, Pochapsky TC, J Mol Biol. 2006 Nov 3;363(4):823-34. Epub 2006 Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16989860 16989860]
One protein, two enzymes., Dai Y, Wensink PC, Abeles RH, J Biol Chem. 1999 Jan 15;274(3):1193-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9880484 9880484]
[[Category: Klebsiella oxytoca]]
[[Category: Klebsiella oxytoca]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Non-heme iron]]
[[Category: Non-heme iron]]
[[Category: Structural entropy]]
[[Category: Structural entropy]]
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