1nzd: Difference between revisions

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[[Image:1nzd.jpg|left|200px]]
{{Seed}}
[[Image:1nzd.png|left|200px]]


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{{STRUCTURE_1nzd|  PDB=1nzd  |  SCENE=  }}  
{{STRUCTURE_1nzd|  PDB=1nzd  |  SCENE=  }}  


'''T4 phage BGT-D100A mutant in complex with UDP-glucose: Form I'''
===T4 phage BGT-D100A mutant in complex with UDP-glucose: Form I===




==Overview==
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T4 phage beta-glucosyltransferase (BGT) is an inverting glycosyltransferase (GT) that transfers glucose from uridine diphospho-glucose (UDP-glucose) to an acceptor modified DNA. BGT belongs to the GT-B structural superfamily, represented, so far, by five different inverting or retaining GT families. Here, we report three high-resolution X-ray structures of BGT and a point mutant solved in the presence of UDP-glucose. The two co-crystal structures of the D100A mutant show that, unlike the wild-type enzyme, this mutation prevents glucose hydrolysis. This strongly indicates that Asp100 is the catalytic base. We obtained the wild-type BGT-UDP-glucose complex by soaking substrate-free BGT crystals. Comparison with a previous structure of BGT solved in the presence of the donor product UDP and an acceptor analogue provides the first model of an inverting GT-B enzyme in which both the donor and acceptor substrates are bound to the active site. The structural analyses support the in-line displacement reaction mechanism previously proposed, locate residues involved in donor substrate specificity and identify the catalytic base.
The line below this paragraph, {{ABSTRACT_PUBMED_12860129}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_12860129}}


==About this Structure==
==About this Structure==
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[[Category: Gt-b]]
[[Category: Gt-b]]
[[Category: Udp-glucose]]
[[Category: Udp-glucose]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:10:28 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:18:37 2008''

Revision as of 00:18, 28 July 2008

File:1nzd.png

Template:STRUCTURE 1nzd

T4 phage BGT-D100A mutant in complex with UDP-glucose: Form I

Template:ABSTRACT PUBMED 12860129

About this Structure

1NZD is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: glucose binding and identification of the catalytic base for a direct displacement mechanism., Lariviere L, Gueguen-Chaignon V, Morera S, J Mol Biol. 2003 Jul 25;330(5):1077-86. PMID:12860129

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