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| {{STRUCTURE_1os7| PDB=1os7 | SCENE= }} | | {{STRUCTURE_1os7| PDB=1os7 | SCENE= }} |
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| '''Crystal structure of TauD with iron, alpha-ketoglutarate and Taurine bound at pH 7.5'''
| | ===Crystal structure of TauD with iron, alpha-ketoglutarate and Taurine bound at pH 7.5=== |
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| ==Overview==
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| The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonate (taurine) to sulfite and aminoacetaldehyde while decomposing alphaKG to succinate and CO(2). This alphaKG dependent dioxygenase is expressed in Escherichia coli under sulfur starvation conditions and allows the cell to utilize taurine, and other similar sulfonates in the environment, as an alternative sulfur source. In this work, we report the structures of the apo and holo forms of TauD to 1.9 A resolution (R(cryst) = 21.2%, R(free) = 24.9%) and 2.5 A resolution (R(cryst) = 22.5%, R(free) = 27.8%), respectively. The models reported herein provide significant new insight into the substrate orientations at the active site and the conformational changes that are induced upon taurine binding. Furthermore, analysis of our crystallographic data coupled with reanalysis of the crystallographic model (resolution = 3.0 A, R(cryst) = 28.1, R(free) = 32.0) presented by Elkins et al. (Biochemistry (2002) 41, 5185-5192) reveals an alternative oligomeric arrangement for the enzyme that is consistent with the conserved primary and secondary structure elements of other alphaKG dependent dioxygenases. | | The line below this paragraph, {{ABSTRACT_PUBMED_12741810}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12741810 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12741810}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Taud]] | | [[Category: Taud]] |
| [[Category: Taurine]] | | [[Category: Taurine]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:13:13 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:23:39 2008'' |
