|
|
| Line 1: |
Line 1: |
| [[Image:2azt.gif|left|200px]] | | {{Seed}} |
| | [[Image:2azt.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2azt| PDB=2azt | SCENE= }} | | {{STRUCTURE_2azt| PDB=2azt | SCENE= }} |
|
| |
|
| '''Crystal structure of H176N mutant of human Glycine N-Methyltransferase'''
| | ===Crystal structure of H176N mutant of human Glycine N-Methyltransferase=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| In the presence of moderate (2-4 M) urea concentrations the tetrameric enzyme, glycine N-methyltransferase (GNMT), dissociates into compact monomers. Higher concentrations of urea (7-8 M) promote complete denaturation of the enzyme. We report here that the H176N mutation in this enzyme, found in humans with hypermethioninaemia, significantly decreases stability of the tetramer, although H176 is located far from the intersubunit contact areas. Dissociation of the tetramer to compact monomers and unfolding of compact monomers of the mutant protein were detected by circular dichroism, quenching of fluorescence emission, size-exclusion chromatography, and enzyme activity. The values of apparent free energy of dissociation of tetramer and of unfolding of compact monomers for the H176N mutant (27.7 and 4.2 kcal/mol, respectively) are lower than those of wild-type protein (37.5 and 6.2 kcal/mol). A 2.7 A resolution structure of the mutant protein revealed no significant difference in the conformation of the protein near the mutated residue.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17660255}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17660255 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_17660255}} |
|
| |
|
| ==Disease== | | ==Disease== |
| Line 32: |
Line 36: |
| [[Category: Wagner, C.]] | | [[Category: Wagner, C.]] |
| [[Category: Glycine n-methyltransferase]] | | [[Category: Glycine n-methyltransferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:40:24 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:50:38 2008'' |
