2tlx: Difference between revisions

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[[Image:2tlx.jpg|left|200px]]
{{Seed}}
[[Image:2tlx.png|left|200px]]


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{{STRUCTURE_2tlx|  PDB=2tlx  |  SCENE=  }}  
{{STRUCTURE_2tlx|  PDB=2tlx  |  SCENE=  }}  


'''THERMOLYSIN (NATIVE)'''
===THERMOLYSIN (NATIVE)===




==Overview==
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Multiple-solvent crystal structure determination (MSCS) allows the position and orientation of bound solvent fragments to be identified by determining the structure of protein crystals soaked in organic solvents. We have extended this technique by the determination of high-resolution crystal structures of thermolysin (TLN), generated from crystals soaked in 2% to 100% isopropanol. The procedure causes only minor changes to the conformation of the protein, and an increasing number of isopropanol interaction sites could be identified as the solvent concentration is increased. Isopropanol occupies all four of the main subsites in the active site, although this was only observed at very high concentrations of isopropanol for three of the four subsites. Analysis of the isopropanol positions shows little correlation with interaction energy computed using a molecular mechanics force field, but the experimentally determined positions of isopropanol are consistent with the structures of known protein-ligand complexes of TLN.
The line below this paragraph, {{ABSTRACT_PUBMED_10651278}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_10651278}}


==About this Structure==
==About this Structure==
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[[Category: Metalloproteinase]]
[[Category: Metalloproteinase]]
[[Category: Organic solvent]]
[[Category: Organic solvent]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:57:57 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:59:31 2008''

Revision as of 00:59, 28 July 2008

File:2tlx.png

Template:STRUCTURE 2tlx

THERMOLYSIN (NATIVE)

Template:ABSTRACT PUBMED 10651278

About this Structure

2TLX is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.

Reference

Locating interaction sites on proteins: the crystal structure of thermolysin soaked in 2% to 100% isopropanol., English AC, Done SH, Caves LS, Groom CR, Hubbard RE, Proteins. 1999 Dec 1;37(4):628-40. PMID:10651278

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