2bm0: Difference between revisions

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[[Image:2bm0.gif|left|200px]]
{{Seed}}
[[Image:2bm0.png|left|200px]]


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{{STRUCTURE_2bm0|  PDB=2bm0  |  SCENE=  }}  
{{STRUCTURE_2bm0|  PDB=2bm0  |  SCENE=  }}  


'''RIBOSOMAL ELONGATION FACTOR G (EF-G) FUSIDIC ACID RESISTANT MUTANT T84A'''
===RIBOSOMAL ELONGATION FACTOR G (EF-G) FUSIDIC ACID RESISTANT MUTANT T84A===




==Overview==
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Fusidic acid (FA) is a steroid antibiotic commonly used against Gram positive bacterial infections. It inhibits protein synthesis by stalling elongation factor G (EF-G) on the ribosome after translocation. A significant number of the mutations conferring strong FA resistance have been mapped at the interfaces between domains G, III and V of EF-G. However, direct information on how such mutations affect the structure has hitherto not been available. Here we present the crystal structures of two mutants of Thermus thermophilus EF-G, G16V and T84A, which exhibit FA hypersensitivity and resistance in vitro, respectively. These mutants also have higher and lower affinity for GTP respectively than wild-type EF-G. The mutations cause significant conformational changes in the switch II loop that have opposite effects on the position of a key residue, Phe90, which undergoes large conformational changes. This correlates with the importance of Phe90 in FA sensitivity reported in previous studies. These structures substantiate the importance of the domain G/domain III/domain V interfaces as a key component of the FA binding site. The mutations also cause subtle changes in the environment of the "P-loop lysine", Lys25. This led us to examine the conformation of the equivalent residue in all structures of translational GTPases, which revealed that EF-G and eEF2 form a group separate from the others and suggested that the role of Lys25 may be different in the two groups.
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{{ABSTRACT_PUBMED_15843024}}


==About this Structure==
==About this Structure==
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[[Category: Switch ii]]
[[Category: Switch ii]]
[[Category: Translation mutation thr84ala]]
[[Category: Translation mutation thr84ala]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:28:22 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:04:14 2008''

Revision as of 01:04, 28 July 2008

File:2bm0.png

Template:STRUCTURE 2bm0

RIBOSOMAL ELONGATION FACTOR G (EF-G) FUSIDIC ACID RESISTANT MUTANT T84A

Template:ABSTRACT PUBMED 15843024

About this Structure

2BM0 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural insights into fusidic acid resistance and sensitivity in EF-G., Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT, J Mol Biol. 2005 May 13;348(4):939-49. PMID:15843024

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