|
|
| Line 1: |
Line 1: |
| [[Image:1mvq.gif|left|200px]] | | {{Seed}} |
| | [[Image:1mvq.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1mvq| PDB=1mvq | SCENE= }} | | {{STRUCTURE_1mvq| PDB=1mvq | SCENE= }} |
|
| |
|
| '''Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose'''
| | ===Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Carbohydrate-protein interactions play a key role in many biological processes. Cramoll is a lectin purified from Cratylia mollis seeds that is taxonomically related to concanavalin A (Con A). Although Cramoll and Con A have the same monosaccharide specificity, they have different glycoprotein binding profiles. We report the primary structure of Cramoll, determined by Edman degradation and mass spectrometry and its 1.77 A crystallographic structure and compare it with the three-dimensional structure of Con A in an attempt to understand how differential binding can be achieved by similar or nearly identical structures. We report here that Cramoll consists of 236 residues, with 82% identity with Con A, and that its topological architecture is essentially identical to Con A, because the Calpha positional differences are below 3.5 A. Cramoll and Con A have identical binding sites for MealphaMan, Mn2+, and Ca2+. However, we observed six substitutions in a groove adjacent to the extended binding site and two in the extended binding site that may explain the differences in binding of oligosaccharides and glycoproteins between Cramoll and Con A.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12966038}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12966038 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_12966038}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| [[Category: Trapani, S.]] | | [[Category: Trapani, S.]] |
| [[Category: Legume lectin]] | | [[Category: Legume lectin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:46:44 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:18:59 2008'' |
