2a8m: Difference between revisions

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[[Image:2a8m.gif|left|200px]]
{{Seed}}
[[Image:2a8m.png|left|200px]]


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{{STRUCTURE_2a8m|  PDB=2a8m  |  SCENE=  }}  
{{STRUCTURE_2a8m|  PDB=2a8m  |  SCENE=  }}  


'''Crystal Structure of Human Taspase1 (T234S mutant)'''
===Crystal Structure of Human Taspase1 (T234S mutant)===




==Overview==
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Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.
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{{ABSTRACT_PUBMED_16216576}}


==About this Structure==
==About this Structure==
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[[Category: Mll]]
[[Category: Mll]]
[[Category: Taspase]]
[[Category: Taspase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:45:01 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:19:19 2008''

Revision as of 01:19, 28 July 2008

File:2a8m.png

Template:STRUCTURE 2a8m

Crystal Structure of Human Taspase1 (T234S mutant)

Template:ABSTRACT PUBMED 16216576

About this Structure

2A8M is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52. PMID:16216576

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