1rtc: Difference between revisions

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[[Image:1rtc.jpg|left|200px]]
{{Seed}}
[[Image:1rtc.png|left|200px]]


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{{STRUCTURE_1rtc|  PDB=1rtc  |  SCENE=  }}  
{{STRUCTURE_1rtc|  PDB=1rtc  |  SCENE=  }}  


'''THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS'''
===THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS===




==Overview==
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The plant cytotoxin ricin is a heterodimer with a cell surface binding (B) chain and an enzymatically active A chain (RTA) known to act as a specific N-glycosidase. RTA must be separated from B chain to attack rRNA. The X-ray structure of ricin has been solved recently; here we report the structure of the isolated A chain expressed from a clone in Escherichia coli. This structure of wild-type rRTA has and will continue to serve as the parent compound for difference Fouriers used to assess the structure of site-directed mutants designed to analyze the mechanism of this medically and commercially important toxin. The structure of the recombinant protein, rRTA, is virtually identical to that seen previously for A chain in the heterodimeric toxin. Some minor conformational changes due to interactions with B chain and to crystal packing differences are described. Perhaps the most significant difference is the presence in rRTA of an additional active site water. This molecule is positioned to act as the ultimate nucleophile in the depurination reaction mechanism proposed by Monzingo and Robertus (1992, J. Mol. Biol. 227, 1136-1145).
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{{ABSTRACT_PUBMED_8453380}}


==About this Structure==
==About this Structure==
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[[Category: Robertus, J D.]]
[[Category: Robertus, J D.]]
[[Category: Glycosidase]]
[[Category: Glycosidase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:53:00 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:19:36 2008''

Revision as of 01:19, 28 July 2008

File:1rtc.png

Template:STRUCTURE 1rtc

THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS

Template:ABSTRACT PUBMED 8453380

About this Structure

1RTC is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.

Reference

Structure of recombinant ricin A chain at 2.3 A., Mlsna D, Monzingo AF, Katzin BJ, Ernst S, Robertus JD, Protein Sci. 1993 Mar;2(3):429-35. PMID:8453380

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