2g7f: Difference between revisions

Line 1:

[[Image:2g7f.~~jpg~~|left|200px]]

[[Image:2g7f.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I~~'''~~

===The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I===

~~==Overview==~~

<!--

The ~~crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and at neutral pH. Crystals grown at pH 4.6 and 6.9 diffracted~~ to ~~1.6 A (on BM01A at~~ the ~~ESRF) and 1.95 A~~ (on ~~a rotating-anode generator), respectively. The structures of the endonuclease were compared with the structure of a homologous enzyme in V. vulnificus. The structures of the V. cholerae enzyme~~ at ~~different pH values are essentially identical to each other and to the V~~. ~~vulnificus enzyme~~. However, interesting features were observed in the solvent structures. Both V. cholerae structures reveal the presence of a chloride ion completely buried within the core of the protein, with the nearest solvent molecule approximately 7 A away. Magnesium, which is essential for catalysis, is ~~present in~~ the ~~structure at neutral pH, but is absent at low pH, and may partly explain the inactivity of the enzyme at lower pH~~.

The line below this paragraph, {{ABSTRACT_PUBMED_17057343}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17057343 is the PubMed ID number.

-->

==About this Structure==

Line 31:

Line 35:

[[Category: Endonuclease i]]

[[Category: Vibrio cholerae nuclease]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4~~ 04:46:31 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:23:25 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2g7f.jpg|left|200px]]
+{{Seed}}
+[[Image:2g7f.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2g7f|  PDB=2g7f  |  SCENE=  }}
-'''The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I'''
+===The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I===
-==Overview==
+<!--
-The crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and at neutral pH. Crystals grown at pH 4.6 and 6.9 diffracted to 1.6 A (on BM01A at the ESRF) and 1.95 A (on a rotating-anode generator), respectively. The structures of the endonuclease were compared with the structure of a homologous enzyme in V. vulnificus. The structures of the V. cholerae enzyme at different pH values are essentially identical to each other and to the V. vulnificus enzyme. However, interesting features were observed in the solvent structures. Both V. cholerae structures reveal the presence of a chloride ion completely buried within the core of the protein, with the nearest solvent molecule approximately 7 A away. Magnesium, which is essential for catalysis, is present in the structure at neutral pH, but is absent at low pH, and may partly explain the inactivity of the enzyme at lower pH.
+The line below this paragraph, {{ABSTRACT_PUBMED_17057343}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17057343 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17057343}}
 ==About this Structure==
@@ Line 31: / Line 35: @@
 [[Category: Endonuclease i]]
 [[Category: Vibrio cholerae nuclease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:46:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:23:25 2008''