2v6e: Difference between revisions

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[[Image:2v6e.~~gif~~|left|200px]]

[[Image:2v6e.png|left|200px]]

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~~'''~~PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA~~'''~~

===PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA===

~~==Overview==~~

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The ~~termini of linear chromosomes are protected by specialized DNA structures known~~ as ~~telomeres that also facilitate the complete replication of DNA ends~~. ~~The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses~~. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase)~~. Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2~~, ~~in complex with~~ the ~~palindromic target DNA~~. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-~~induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.~~

The line below this paragraph, {{ABSTRACT_PUBMED_17889664}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17889664 is the PubMed ID number.

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==About this Structure==

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[[Category: Protelomerase]]

[[Category: Resolvase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 18~~:16:33 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:28:03 2008''

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-[[Image:2v6e.gif|left|200px]]
+{{Seed}}
+[[Image:2v6e.png|left|200px]]
 <!--
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 {{STRUCTURE_2v6e|  PDB=2v6e  |  SCENE=  }}
-'''PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA'''
+===PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA===
-==Overview==
+<!--
-The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.
+The line below this paragraph, {{ABSTRACT_PUBMED_17889664}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17889664 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17889664}}
 ==About this Structure==
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 [[Category: Protelomerase]]
 [[Category: Resolvase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:16:33 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:28:03 2008''