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| {{STRUCTURE_2f9s| PDB=2f9s | SCENE= }} | | {{STRUCTURE_2f9s| PDB=2f9s | SCENE= }} |
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| '''2nd Crystal Structure Of A Soluble Domain Of ResA In The Oxidised Form'''
| | ===2nd Crystal Structure Of A Soluble Domain Of ResA In The Oxidised Form=== |
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| ==Overview==
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| The covalent attachment of heme cofactors to the apo-polypeptides via thioether bonds is unique to the maturation of c-type cytochromes. A number of thiol-disulfide oxidoreductases prepare the apocytochrome for heme insertion in system I and II cytochrome c maturation. Although most thiol-disulfide oxidoreductases are nonspecific, the less common, specific thiol-disulfide oxidoreductases may be key to directing the usage of electrons. Here we demonstrate that unlike other thiol-disulfide oxidoreductases, the protein responsible for reducing oxidized apocytochrome c in Bacillus subtilis, ResA, is specific for cytochrome c550 and utilizes alternate conformations to recognize redox partners. We report solution NMR evidence that ResA undergoes a redox-dependent conformational change between oxidation states, as well as data showing that ResA utilizes a surface cavity present only in the reduced state to recognize a peptide derived from cytochrome c550. Finally, we confirm that ResA is a specific thiol-disulfide oxidoreductase by comparing its reactivity to our mimetic peptide with its reactivity to oxidized glutathione, a nonspecific substrate. This study biochemically demonstrates the specificity of this thiol-disulfide oxidoreductase and enables us to outline a structural mechanism of regulating the usage of electrons in a thiol-disulfide oxidoreductase system. | | The line below this paragraph, {{ABSTRACT_PUBMED_16537372}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16537372 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16537372}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Wu, Q.]] | | [[Category: Wu, Q.]] |
| [[Category: Thioredoxin-like protein]] | | [[Category: Thioredoxin-like protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:38:25 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:55:02 2008'' |
