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| {{STRUCTURE_1omx| PDB=1omx | SCENE= }} | | {{STRUCTURE_1omx| PDB=1omx | SCENE= }} |
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| '''Crystal structure of mouse alpha-1,4-N-acetylhexosaminyltransferase (EXTL2)'''
| | ===Crystal structure of mouse alpha-1,4-N-acetylhexosaminyltransferase (EXTL2)=== |
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| ==Overview==
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| EXTL2, an alpha1,4-N-acetylhexosaminyltransferase, catalyzes the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta1-3[galactose]beta1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. We have solved the x-ray crystal structure of the catalytic domain of mouse EXTL2 in the apo-form and with donor substrates UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine. In addition, a structure of the ternary complex with UDP and the acceptor substrate analog [glucuronic acid]beta1-3[galactose]beta1-O-naphthalenemethanol has been determined. These structures reveal three highly conserved residues, Asn-243, Asp-246, and Arg-293, located at the active site. Mutation of these residues greatly decreases the activity. In the ternary complex, an interaction exists between the beta-phosphate of the UDP leaving group and the acceptor hydroxyl of the substrate that may play a functional role in catalysis. These structures represent the first structures from the exostosin gene family and provide important insight into the mechanisms of alpha1,4-N-acetylhexosaminyl transfer in heparan biosynthesis.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12562774}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12562774 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12562774}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Dxd motif]] | | [[Category: Dxd motif]] |
| [[Category: Rossmann fold]] | | [[Category: Rossmann fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:02:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:11:37 2008'' |
