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| [[Image:1yuh.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1yuh| PDB=1yuh | SCENE= }} | | {{STRUCTURE_1yuh| PDB=1yuh | SCENE= }} |
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| '''FAB FRAGMENT'''
| | ===FAB FRAGMENT=== |
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| ==Overview==
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| Affinity maturation of the immune response to nitrophenol-containing antigens has been extensively investigated. Significant strides made during the past several years with the advent of PCR technology have provided a wealth of biochemical knowledge. Structural investigations of the phenomena have however been limited. We have determined the three-dimensional structure of the Fab fragment of 88C6/12, an anti-4-hydroxy-3-nitrophenyl acetic acid antibody complexed with the immunizing hapten and with a heteroclitic iodinated hapten. The crystallographic structure of the complexes reveals that the binding is stabilized by a number of hydrogen bonds and extensive van der Waals interactions between the hapten and the antibody. In addition, the Fab binding pocket contains a region of positive electrostatic potential well suited for interaction with the predominant resonance form of the nitrophenyl ring system. The observed heteroclicity towards the iodinated hapten is not a direct result of iodine-protein interactions, but results from the enhanced stability in the iodinated ring of the resonance form that binds the antibody. In addition this investigation provides a rationale for the strong preference for the substitution in the heavy chain from the germ-line gene encoded Trp 33 to Leu 33 in the mature anti-nitrophenol response.
| | The line below this paragraph, {{ABSTRACT_PUBMED_8544863}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8544863 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8544863}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| [[Category: Lambda light chain]] | | [[Category: Lambda light chain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:48:23 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:16:11 2008'' |
