2poo: Difference between revisions

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[[Image:2poo.jpg|left|200px]]
{{Seed}}
[[Image:2poo.png|left|200px]]


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{{STRUCTURE_2poo|  PDB=2poo  |  SCENE=  }}  
{{STRUCTURE_2poo|  PDB=2poo  |  SCENE=  }}  


'''THERMOSTABLE PHYTASE IN FULLY CALCIUM LOADED STATE'''
===THERMOSTABLE PHYTASE IN FULLY CALCIUM LOADED STATE===




==Overview==
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A novel bacterial phytase from a Bacillus amyloliquefaciens strain was crystallized using the hanging-drop vapour-diffusion method. The amino-acid sequence of the enzyme does not show any homology to those of other known phytases or phosphatases, with the exception of a phytase from Bacillus subtilis. The enzyme exhibits a thermal stability which is strongly dependent on calcium ions. High-quality single crystals of the enzyme in the absence of calcium ions were obtained using a precipitant solution containing 20% 2-methyl-2, 4-pentanediol and 0.1 M MES (pH 6.5). Native diffraction data to 2.0 A resolution were obtained from a flash-frozen crystal at 110 K using a rotating-anode X-ray source. The crystals belong to space group P212121 with unit-cell dimensions a = 50.4, b = 64.1, c = 104. 2 A and contain one monomer per asymmetric unit. Structure determination using heavy-atom derivative crystals is in progress, along with an effort to crystallize the calcium ion bound form of the enzyme.
The line below this paragraph, {{ABSTRACT_PUBMED_10089471}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10089471 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10089471}}


==About this Structure==
==About this Structure==
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[[Category: Calcium loaded state]]
[[Category: Calcium loaded state]]
[[Category: Thermostable phytase]]
[[Category: Thermostable phytase]]
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