2f8e: Difference between revisions

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[[Image:2f8e.gif|left|200px]]
{{Seed}}
[[Image:2f8e.png|left|200px]]


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{{STRUCTURE_2f8e|  PDB=2f8e  |  SCENE=  }}  
{{STRUCTURE_2f8e|  PDB=2f8e  |  SCENE=  }}  


'''Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with uridylylated VPg protein'''
===Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with uridylylated VPg protein===




==Overview==
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Picornavirus RNA replication is initiated by the covalent attachment of a UMP molecule to the hydroxyl group of a tyrosine in the terminal protein VPg. This reaction is carried out by the viral RNA-dependent RNA polymerase (3D). Here, we report the X-ray structure of two complexes between foot-and-mouth disease virus 3D, VPg1, the substrate UTP and divalent cations, in the absence and in the presence of an oligoadenylate of 10 residues. In both complexes, VPg fits the RNA binding cleft of the polymerase and projects the key residue Tyr3 into the active site of 3D. This is achieved by multiple interactions with residues of motif F and helix alpha8 of the fingers domain and helix alpha13 of the thumb domain of the polymerase. The complex obtained in the presence of the oligoadenylate showed the product of the VPg uridylylation (VPg-UMP). Two metal ions and the catalytic aspartic acids of the polymerase active site, together with the basic residues of motif F, have been identified as participating in the priming reaction.
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{{ABSTRACT_PUBMED_16456546}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16456546 16456546]
The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16456546 16456546]
Structure of foot-and-mouth disease virus RNA-dependent RNA polymerase and its complex with a template-primer RNA., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, J Biol Chem. 2004 Nov 5;279(45):47212-21. Epub 2004 Aug 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15294895 15294895]
[[Category: Foot-and-mouth disease virus c]]
[[Category: Foot-and-mouth disease virus c]]
[[Category: RNA-directed RNA polymerase]]
[[Category: RNA-directed RNA polymerase]]
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[[Category: Protein primer]]
[[Category: Protein primer]]
[[Category: Rna-dependent rna polymerase ,vpg protein]]
[[Category: Rna-dependent rna polymerase ,vpg protein]]
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