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| {{STRUCTURE_1v7x| PDB=1v7x | SCENE= }} | | {{STRUCTURE_1v7x| PDB=1v7x | SCENE= }} |
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| '''Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate'''
| | ===Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate=== |
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| ==Overview==
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| Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl transferase family 36 (GT-36), and catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. As the first known structures of a GT-36 enzyme, we determined the crystal structure of ChBP in a ternary complex with GlcNAc and SO(4). It is also the first structures of an inverting phosphorolytic enzyme in a complex with a sugar and a sulfate ion, and reveals a pseudo-ternary complex structure of enzyme-sugar-phosphate. ChBP comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain, constituting a distinctive structure among GT families. Instead, it shows significant structural similarity with glycoside hydrolase (GH) enzymes, glucoamylases (GH-15), and maltose phosphorylase (GH-65) in clan GH-L. The structural similarity reported here, together with distant sequence similarities between ChBP and GHs, led to the reclassification of family GT-36 into a novel GH family, namely GH-94.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15274915}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15274915 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15274915}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Wakagi, T.]] | | [[Category: Wakagi, T.]] |
| [[Category: Beta-sandwich]] | | [[Category: Beta-sandwich]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:11:38 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:30:12 2008'' |
