1v9h: Difference between revisions

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[[Image:1v9h.jpg|left|200px]]
{{Seed}}
[[Image:1v9h.png|left|200px]]


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{{STRUCTURE_1v9h|  PDB=1v9h  |  SCENE=  }}  
{{STRUCTURE_1v9h|  PDB=1v9h  |  SCENE=  }}  


'''Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP'''
===Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP===




==Overview==
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The ribonuclease MC1 (RNase MC1) from the seeds of the bitter gourd belongs to the RNase T2 family. We evaluated the contribution of 11 amino acids conserved in the RNase T2 family to protein folding of RNase MC1. Thermal unfolding experiments showed that substitution of Tyr(101), Phe(102), Ala(105), and Phe(190) resulted in a significant decrease in themostability; the T(m) values were 47-58 degrees C compared to that for the wild type (64 degrees C). Mutations of Pro(125), Gly(127), Gly(144), and Val(165) caused a moderate decrease in thermostability (T(m): 60-62 degrees C). In contrast, mutations of Asp(107) and Gly(173) did little effect on thermostability. The contribution of Tyr(101), Phe(102), Pro(125), and Gly(127) to protein stability was further corroborated by means of Gdn-HCl unfolding and protease digestions. Taken together, it appeared that Tyr(101), Phe(102), Ala(105), Pro(125), Gly(127), Gly(144), Leu(162), Val(165), and Phe(190) conserved in the RNase T2 family play an important role in the stability of the proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 15322360 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15322360}}


==About this Structure==
==About this Structure==
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[[Category: Nucleic acid]]
[[Category: Nucleic acid]]
[[Category: Rna]]
[[Category: Rna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:15:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:34:25 2008''

Revision as of 02:34, 28 July 2008

File:1v9h.png

Template:STRUCTURE 1v9h

Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP

Template:ABSTRACT PUBMED 15322360

About this Structure

1V9H is a Single protein structure of sequence from Momordica charantia. Full crystallographic information is available from OCA.

Reference

Amino acids conserved at the C-terminal half of the ribonuclease T2 family contribute to protein stability of the enzymes., Kimura K, Numata T, Kakuta Y, Kimura M, Biosci Biotechnol Biochem. 2004 Aug;68(8):1748-57. PMID:15322360

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