1wbf: Difference between revisions

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[[Image:1wbf.jpg|left|200px]]
{{Seed}}
[[Image:1wbf.png|left|200px]]


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{{STRUCTURE_1wbf|  PDB=1wbf  |  SCENE=  }}  
{{STRUCTURE_1wbf|  PDB=1wbf  |  SCENE=  }}  


'''WINGED BEAN LECTIN, SACCHARIDE FREE FORM'''
===WINGED BEAN LECTIN, SACCHARIDE FREE FORM===




==Overview==
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The crystal structure of the saccharide-free form of the basic form of winged-bean agglutinin (WBAI) has been solved by the molecular-replacement method and refined at 2.3 A resolution. The final R factor is 19.7% for all data in the resolution range 8.0-2.3 A. The asymmetric unit contains two half-dimers, each located on a crystallographic twofold axis. The structure of the saccharide-free form is compared with that of the complex of WBAI with methyl-alpha-D-galactoside. The complex is composed of two dimers in the asymmetric unit. The intersubunit interactions in the dimer are nearly identical in the two structures. The binding site of the saccharide-free structure contains three ordered water molecules at positions similar to those of the hydroxyl groups of the carbohydrate which are hydrogen bonded to the protein. Superposition of the saccharide-binding sites of the two structures shows that the major changes involve expulsion of these ordered water molecules and a shift of about 0.6 A of the main-chain atoms of the variable loop.
The line below this paragraph, {{ABSTRACT_PUBMED_10089310}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10089310 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10089310}}


==About this Structure==
==About this Structure==
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[[Category: Protein crystallography]]
[[Category: Protein crystallography]]
[[Category: Saccharide free form]]
[[Category: Saccharide free form]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:25:13 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:11:13 2008''

Revision as of 03:11, 28 July 2008

File:1wbf.png

Template:STRUCTURE 1wbf

WINGED BEAN LECTIN, SACCHARIDE FREE FORM

Template:ABSTRACT PUBMED 10089310

About this Structure

1WBF is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.

Reference

Structure of basic winged-bean lectin and a comparison with its saccharide-bound form., Manoj N, Srinivas VR, Suguna K, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310

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