1xyd: Difference between revisions

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[[Image:1xyd.gif|left|200px]]
{{Seed}}
[[Image:1xyd.png|left|200px]]


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{{STRUCTURE_1xyd|  PDB=1xyd  |  SCENE=  }}  
{{STRUCTURE_1xyd|  PDB=1xyd  |  SCENE=  }}  


'''NMR Solution Structure of Rat Zinc-Calcium-S100B, 20 Structures'''
===NMR Solution Structure of Rat Zinc-Calcium-S100B, 20 Structures===




==Overview==
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The EF-hand calcium-binding protein S100B also binds one zinc ion per subunit with a relatively high affinity (K(d) approximately 90 nM) [Wilder et al., (2003) Biochemistry 42, 13410-13421]. In this study, the structural characterization of zinc binding to calcium-loaded S100B was examined using high-resolution NMR techniques, including structural characterization of this complex in solution at atomic resolution. As with other S100 protein structures, the quaternary structure of Zn(2+)-Ca(2+)-bound S100B was found to be dimeric with helices H1, H1', H4, and H4' forming an X-type four-helix bundle at the dimer interface. NMR data together with mutational analyses are consistent with Zn(2+) coordination arising from His-15 and His-25 of one S100B subunit and from His-85 and Glu-89 of the other subunit. The addition of Zn(2+) was also found to extend helices H4 and H4' three to four residues similar to what was previously observed with the binding of target proteins to S100B. Furthermore, a kink in helix 4 was observed in Zn(2+)-Ca(2+)-bound S100B that is not in Ca(2+)-bound S100B. These structural changes upon Zn(2+)-binding could explain the 5-fold increase in affinity that Zn(2+)-Ca(2+)-bound S100B has for peptide targets such as the TRTK peptide versus Ca(2+)-bound S100B. There are also changes in the relative positioning of the two EF-hand calcium-binding domains and the respective helices comprising these EF-hands. Changes in conformation such as these could contribute to the order of magnitude higher affinity that S100B has for calcium in the presence of Zn(2+).
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{{ABSTRACT_PUBMED_15823027}}


==About this Structure==
==About this Structure==
1XYD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XYD OCA].  
1XYD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XYD OCA].  


==Reference==
==Reference==
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[[Category: Wilder, P T.]]
[[Category: Wilder, P T.]]
[[Category: Metal binding protein]]
[[Category: Metal binding protein]]
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Revision as of 03:33, 28 July 2008

File:1xyd.png

Template:STRUCTURE 1xyd

NMR Solution Structure of Rat Zinc-Calcium-S100B, 20 Structures

Template:ABSTRACT PUBMED 15823027

About this Structure

1XYD is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Solution structure of zinc- and calcium-bound rat S100B as determined by nuclear magnetic resonance spectroscopy., Wilder PT, Varney KM, Weiss MB, Gitti RK, Weber DJ, Biochemistry. 2005 Apr 19;44(15):5690-702. PMID:15823027

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