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| [[Image:2c50.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2c50| PDB=2c50 | SCENE= }} | | {{STRUCTURE_2c50| PDB=2c50 | SCENE= }} |
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| '''MS2-RNA HAIRPIN (A-5) COMPLEX'''
| | ===MS2-RNA HAIRPIN (A-5) COMPLEX=== |
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| ==Overview==
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| We have determined the X-ray structures of six MS2 RNA hairpin-coat-protein complexes having five different substitutions at the hairpin loop base -5. This is a uracil in the wild-type hairpin and contacts the coat protein both by stacking on to a tyrosine side chain and by hydrogen bonding to an asparagine side chain. The RNA consensus sequence derived from coat protein binding studies with natural sequence variants suggested that the -5 base needs to be a pyrimidine for strong binding. The five -5 substituents used in this study were 5-bromouracil, pyrimidin-2-one, 2-thiouracil, adenine, and guanine. The structure of the 5-bromouracil complex was determined to 2.2 A resolution, which is the highest to date for any MS2 RNA-protein complex. All the complexes presented here show very similar conformations, despite variation in affinity in solution. The results suggest that the stacking of the -5 base on to the tyrosine side chain is the most important driving force for complex formation. A number of hydrogen bonds that are present in the wild-type complex are not crucial for binding, as they are missing in one or more of the complexes. The results also reveal the flexibility of this RNA-protein interface, with respect to functional group variation, and may be generally applicable to other RNA-protein complexes.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11720290}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11720290 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11720290}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Virus coat protein]] | | [[Category: Virus coat protein]] |
| [[Category: Virus/viral protein/rna]] | | [[Category: Virus/viral protein/rna]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:15:32 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:57:09 2008'' |
