2f7t: Difference between revisions

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[[Image:2f7t.gif|left|200px]]
{{Seed}}
[[Image:2f7t.png|left|200px]]


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{{STRUCTURE_2f7t|  PDB=2f7t  |  SCENE=  }}  
{{STRUCTURE_2f7t|  PDB=2f7t  |  SCENE=  }}  


'''Crystal structure of the catalytic domain of Mos1 mariner transposase'''
===Crystal structure of the catalytic domain of Mos1 mariner transposase===




==Overview==
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We present the crystal structure of the catalytic domain of Mos1 transposase, a member of the Tc1/mariner family of transposases. The structure comprises an RNase H-like core, bringing together an aspartic acid triad to form the active site, capped by N- and C-terminal alpha-helices. We have solved structures with either one Mg2+ or two Mn2+ ions in the active site, consistent with a two-metal mechanism for catalysis. The lack of hairpin-stabilizing structural motifs is consistent with the absence of a hairpin intermediate in Mos1 excision. We have built a model for the DNA-binding domain of Mos1 transposase, based on the structure of the bipartite DNA-binding domain of Tc3 transposase. Combining this with the crystal structure of the catalytic domain provides a model for the paired-end complex formed between a dimer of Mos1 transposase and inverted repeat DNA. The implications for the mechanisms of first and second strand cleavage are discussed.
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{{ABSTRACT_PUBMED_16511570}}


==About this Structure==
==About this Structure==
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[[Category: Ddd motif]]
[[Category: Ddd motif]]
[[Category: Rnase-h like fold]]
[[Category: Rnase-h like fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:33:51 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:11:58 2008''

Revision as of 04:11, 28 July 2008

File:2f7t.png

Template:STRUCTURE 2f7t

Crystal structure of the catalytic domain of Mos1 mariner transposase

Template:ABSTRACT PUBMED 16511570

About this Structure

2F7T is a Single protein structure of sequence from Drosophila mauritiana. Full crystallographic information is available from OCA.

Reference

Mechanism of Mos1 transposition: insights from structural analysis., Richardson JM, Dawson A, O'Hagan N, Taylor P, Finnegan DJ, Walkinshaw MD, EMBO J. 2006 Mar 22;25(6):1324-34. Epub 2006 Mar 2. PMID:16511570

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