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| {{STRUCTURE_1pxu| PDB=1pxu | SCENE= }} | | {{STRUCTURE_1pxu| PDB=1pxu | SCENE= }} |
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| '''Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution'''
| | ===Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution=== |
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| ==Overview==
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| Agrin is a key organizer for postsynaptic differentiation at the neuromuscular junction (NMJ). This activity requires the binding of agrin to the synaptic basal lamina via its N-terminal (NtA) domain. It has been suggested that this binding is mediated by conserved amino acids in the gamma 1 chain of laminin. Here, we report the crystal structure of chicken NtA expressed in eukaryotic HEK293 cells. In contrast to the previously published structure [Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Schumacher, B., Frank, S., Ruegg, M.A., Engel, J., Kammerer, R.A., 2001. The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat. Struct. Biol., 8, 705-709.], which was derived from the NtA domain expressed in E. coli, the new data show that the N-terminal tail region (amino acid residues Asn1-Arg5) is highly structured. Moreover, the disulfide bridge between Cys2 and Cys74 was also present. In addition, we show that the binding of NtA requires the gamma 1 chain of laminin and is not greatly affected by the composition of beta chains. These results confirm a model of the NtA-laminin complex where conserved amino acids in the gamma 1 chain are prerequisite for the binding to agrin and they further emphasize that the source of protein can be critical in structure determination.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15694127}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15694127 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15694127}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Stetefeld, J.]] | | [[Category: Stetefeld, J.]] |
| [[Category: Agrin]] | | [[Category: Agrin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:37:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:15:19 2008'' |
