2jul: Difference between revisions

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[[Image:2jul.jpg|left|200px]]
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{{STRUCTURE_2jul|  PDB=2jul  |  SCENE=  }}  
{{STRUCTURE_2jul|  PDB=2jul  |  SCENE=  }}  


'''NMR Structure of DREAM'''
===NMR Structure of DREAM===




==Overview==
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DREAM (calsenilin/KChIP3) is an EF-hand calcium-binding protein that binds to specific DNA sequences and regulates Ca2+-induced transcription of prodynorphin and c-fos genes. Here, we present the atomic-resolution structure of Ca2+-bound DREAM in solution determined by nuclear magnetic resonance (NMR) spectroscopy. Pulsed-field gradient NMR diffusion experiments and 15N NMR relaxation analysis indicate that Ca2+-bound DREAM forms a stable dimer in solution. The structure of the first 77 residues from the N-terminus could not be determined by our NMR analysis. The C-terminal DREAM structure (residues 78-256) contains four EF-hand motifs arranged in a tandem linear array, similar to that seen in KChIP1, recoverin, and other structures of the neuronal calcium sensor (NCS) branch of the calmodulin superfamily. Mg2+ is bound at the second EF-hand, whereas Ca2+ is bound functionally at the third and fourth sites. The first and second EF-hands form an exposed hydrophobic groove on the protein surface lined by side-chain atoms of L96, F100, F114, I117, Y118, F121, F122, Y151, L155, L158, and L159 that are highly conserved in all NCS proteins. An exposed leucine near the C-terminus (L251) is suggested to form intermolecular contacts with leucine residues in the hydrophobic groove (L155, L158, and L159). Positively charged side chains of Arg and Lys (Lys87, Lys90, Lys91, Arg98, Lys101, Arg160, and Lys166) are clustered on one side of the protein surface and may mediate electrostatic contacts with DNA targets. We propose that Ca2+-induced dimerization of DREAM may partially block the putative DNA-binding site, which may suggest as to how Ca2+ abolishes DREAM binding to DNA to activate the transcription of prodynorphin and other downstream genes in pain control.
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==About this Structure==
==About this Structure==
2JUL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUL OCA].  
2JUL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUL OCA].  


==Reference==
==Reference==
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[[Category: Transport]]
[[Category: Transport]]
[[Category: Voltage-gated channel]]
[[Category: Voltage-gated channel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:37:41 2008''
 
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Revision as of 04:38, 28 July 2008

File:2jul.png

Template:STRUCTURE 2jul

NMR Structure of DREAM

Template:ABSTRACT PUBMED 18201103

About this Structure

2JUL is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

NMR structure of DREAM: Implications for Ca(2+)-dependent DNA binding and protein dimerization., Lusin JD, Vanarotti M, Li C, Valiveti A, Ames JB, Biochemistry. 2008 Feb 26;47(8):2252-64. Epub 2008 Jan 18. PMID:18201103

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