1w53: Difference between revisions

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[[Image:1w53.gif|left|200px]]
{{Seed}}
[[Image:1w53.png|left|200px]]


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{{STRUCTURE_1w53|  PDB=1w53  |  SCENE=  }}  
{{STRUCTURE_1w53|  PDB=1w53  |  SCENE=  }}  


'''KINASE RECRUITMENT DOMAIN OF THE STRESS PHOSPHATASE RSBU'''
===KINASE RECRUITMENT DOMAIN OF THE STRESS PHOSPHATASE RSBU===




==Overview==
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RsbU is a positive regulator of the activity of sigmaB, the general stress-response sigma factor of Gram+ microorganisms. The N-terminal domain of this protein has no significant sequence homology with proteins of known function, whereas the C-terminal domain is similar to the catalytic domains of PP2C-type phosphatases. The phosphatase activity of RsbU is stimulated greatly during the response to stress by associating with a kinase, RsbT. This association leads to the induction of sigmaB activity. Here we present data on the activation process and demonstrate in vivo that truncations in the N-terminal region of RsbU are deleterious for the activation of RsbU. This conclusion is supported by comparisons of the phosphatase activities of full-length and a truncated form of RsbU in vitro. Our determination of the crystal structure of the N-terminal domain of RsbU from Bacillus subtilis reveals structural similarities to the regulatory domains from ubiquitous protein phosphatases and a conserved domain of sigma-factors, illuminating the activation processes of phosphatases and the evolution of "partner switching." Finally, the molecular basis of kinase recruitment by the RsbU phosphatase is discussed by comparing RsbU sequences from bacteria that either possess or lack RsbT.
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{{ABSTRACT_PUBMED_15263010}}


==About this Structure==
==About this Structure==
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[[Category: Phosphatase]]
[[Category: Phosphatase]]
[[Category: Stress]]
[[Category: Stress]]
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Revision as of 04:42, 28 July 2008

File:1w53.png

Template:STRUCTURE 1w53

KINASE RECRUITMENT DOMAIN OF THE STRESS PHOSPHATASE RSBU

Template:ABSTRACT PUBMED 15263010

About this Structure

1W53 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Functional and structural characterization of RsbU, a stress signaling protein phosphatase 2C., Delumeau O, Dutta S, Brigulla M, Kuhnke G, Hardwick SW, Volker U, Yudkin MD, Lewis RJ, J Biol Chem. 2004 Sep 24;279(39):40927-37. Epub 2004 Jul 19. PMID:15263010

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