2aje: Difference between revisions

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[[Image:2aje.gif|left|200px]]
{{Seed}}
[[Image:2aje.png|left|200px]]


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{{STRUCTURE_2aje|  PDB=2aje  |  SCENE=  }}  
{{STRUCTURE_2aje|  PDB=2aje  |  SCENE=  }}  


'''Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain'''
===Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain===




==Overview==
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The double-stranded telomeric repeat-binding protein (TRP) AtTRP1 is isolated from Arabidopsis thaliana. Using gel retardation assays, we defined the C-terminal 97 amino acid residues, Gln464 to Val560 (AtTRP1(464-560)), as the minimal structured telomeric repeat-binding domain. This region contains a typical Myb DNA-binding motif and a C-terminal extension of 40 amino acid residues. The monomeric AtTRP1(464-560) binds to a 13-mer DNA duplex containing a single repeat of an A.thaliana telomeric DNA sequence (GGTTTAG) in a 1:1 complex, with a K(D) approximately 10(-6)-10(-7) M. Nuclear magnetic resonance (NMR) examination revealed that the solution structure of AtTRP1(464-560) is a novel four-helix tetrahedron rather than the three-helix bundle structure found in typical Myb motifs and other TRPs. Binding of the 13-mer DNA duplex to AtTRP1(464-560) induced significant chemical shift perturbations of protein amide resonances, which suggests that helix 3 (H3) and the flexible loop connecting H3 and H4 are essential for telomeric DNA sequence recognition. Furthermore, similar to that in hTRF1, the N-terminal arm likely contributes to or stabilizes DNA binding. Sequence comparisons suggested that the four-helix structure and the involvement of the loop residues in DNA binding may be features unique to plant TRPs.
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{{ABSTRACT_PUBMED_16337232}}


==About this Structure==
==About this Structure==
2AJE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJE OCA].  
2AJE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJE OCA].  


==Reference==
==Reference==
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[[Category: Telomere]]
[[Category: Telomere]]
[[Category: Trp]]
[[Category: Trp]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:07:27 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:43:33 2008''

Revision as of 04:43, 28 July 2008

File:2aje.png

Template:STRUCTURE 2aje

Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain

Template:ABSTRACT PUBMED 16337232

About this Structure

2AJE is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix., Sue SC, Hsiao HH, Chung BC, Cheng YH, Hsueh KL, Chen CM, Ho CH, Huang TH, J Mol Biol. 2006 Feb 10;356(1):72-85. Epub 2005 Nov 22. PMID:16337232

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