1u7g: Difference between revisions

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{{STRUCTURE_1u7g|  PDB=1u7g  |  SCENE=  }}  
{{STRUCTURE_1u7g|  PDB=1u7g  |  SCENE=  }}  


'''Crystal Structure of Ammonia Channel AmtB from E. Coli'''
===Crystal Structure of Ammonia Channel AmtB from E. Coli===




==Overview==
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The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.
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{{ABSTRACT_PUBMED_15361618}}


==About this Structure==
==About this Structure==
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[[Category: Right handed helical bundle]]
[[Category: Right handed helical bundle]]
[[Category: Transmembrane helice]]
[[Category: Transmembrane helice]]
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Revision as of 04:50, 28 July 2008

File:1u7g.png

Template:STRUCTURE 1u7g

Crystal Structure of Ammonia Channel AmtB from E. Coli

Template:ABSTRACT PUBMED 15361618

About this Structure

1U7G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:15361618

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